The present study compared the effects of incorporating traditional sprint interval training (SIT) or basketball-specific SIT (SSIT) into typical off-season training of male basketball players. Adaptations to and effect size (EF) of interventions on aerobic fitness [evaluated using Yo-Yo intermittent recovery test level-1 (Yo-Yo IR1)], change of direction [T-test (TT) and Illinois agility test (IAT)], vertical jump (VJ), standing long jump (SLJ), linear speed, maximal strength [one repetition maximum test in leg press (1RM_LP)], and hormonal status were examined. Male athletes (age = 25.7 ± 2.0 years; height = 188.1 ± 7.9 cm; body mass = 85.9 ± 8.0 kg) were randomly assigned to one of three groups of SIT (n = 10): three sets of 10 × 15 sec all-out intervals with 1:1 recovery between bouts and a 3-min recovery between sets; SSIT (n = 10): the same intervals as SIT + basketball-specific ball drills while running; and CON (n = 10): two sessions per week of regular basketball technical and tactical drills. SIT and SSIT resulted in significant changes compared with baseline in maximal oxygen uptake (4.9%, ES = 2.22 vs. 6%, ES = 2.57), TT (-1.8%, ES =-0.46 vs. -2.7%, ES = -1.14), IAT (-4.5%, ES = -2.01 vs. -5.4%, ES = -1.93), VJ (7.5%, ES = 0.58 vs. 12%, ES = 0.95), linear sprint time (-2.9%, ES = -0.32 vs. -4.3%, ES = -0.69), Yo-Yo IR1 (18.5%, ES = 2.19 vs. 23.7%, ES = 2.56), serum testosterone (28%, ES = 1.52 vs. 29.7%, ES = 1.59), and cortisol (-6.53%, ES = -0.37 vs. -12.06%, ES = -0.64). Incorporating SIT and SSIT into typical off-season basketball training triggers adaptive mechanisms that enhance aerobic and anaerobic performance in male basketball players. The effect size values indicate more significant effects of SSIT than SIT in most physiological and sport-specific adaptations. Such a superior effect could be attributed to the more basketball-specific movement pattern of the SSIT. Such interventions can be used by the coaches and athletes for designing the training load and for better training adaptations throughout the training seasons and competition periods.

• Incorporating SIT and SSIT into typical off-season basketball training triggers adaptive mechanisms that enhance aerobic and anaerobic performance in male basketball players.

• The effect size values indicate more significant effects of SSIT than SIT in most physiological and sport-specific adaptations. Such a superior effect could be attributed to the sport-specific movement pattern of the SSIT.

• The observed alterations in hormonal responses indicate an anabolic adaptation to this intervention, signifying a positive and advantageous training response.

• The integration SIT into routine basketball training offers athletes and coaches an opportunity to harness its potential to optimize training quality and efficiency of their training. This integration stimulates favorable physiological changes, culminating in notable enhancements in athletic performance.